Abstract In the present study,HPLCFLD was used to examine the aflatoxin B1 derivativeB2ain food,with excitation and fluorescence wave-lengths 365nm and 450nm respectively,methanol/H2O=11 in the mobile phase and a flow rate 10ml/min,1254mm Hypersil column packed with ODS5um. A working curve showing a direct ratio (r=1.000) of the concentration against the peak area of AFTB1 was obtained.The method proved to be satisfactory in precision,accuracy and sensitivity,with CV=25%,recovery rate 95% ̄101% and detection limit 02ng.
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