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| Studies on the Extraction Technique of Superoxide Dismutase from Sheep Blood——The Influence of Thermal Denatureation Temperature and Time and Thermal Change Method on Extraction of SOD,and the Selection of Optimal Technique Condition |
| YU Ying;XU Guihua |
| Food Department of Agriculture College of Ningxia University ,Yinchuan 750021, China |
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Abstract Objective: To study the effect of thermal denaturation temperature and thermal denaturation time and thermal change method on extraction of SOD, and to define its optimal technique parameter. Method: Orthogonal experiment design was adopted results: From synthetical analysis of the SOD activity and yield productive. The optimal combination of orthogonal experiment is A2B2C2,that is, thethermal denatureation temperature is 55℃;The thermal denatureation time is 15 min; the amount of 0.01mol/l CuCl2 addition is 2%. Conclusion: Thermal denaturation is one of the most importance in extracting SOD from sheep blood, higher purity SOD, whose activity exceeds 1 000 U/mg protein was reached by this technique.
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2009, Vol. 23 
