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| Optimization of Conditions for Heparin Sodium Partitioning and Purification from Yak's Lung |
| CHENG Linkun;SONG Huanlu |
| College of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048） |
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Abstract The yak's lung was dissolved using hydrolytic enzyme; Heparin sodium was purified by enzyme hydrolyzing combined with oxidation by hydrogen peroxide. Azure A color method was used to determine its potency. The optimal extracting conditions obtained by L9(34) orthogonal experiment were that papain 1:500 , papain enzyme hydrolyzing time 3 hours, trypsin 1:200 , hydrogen peroxide 1%. The potency of the final product under these conditions was 131 U/mg and yield over 1.92%.
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2010, Vol. 24 
