Abstract：A novel low-calorie fat substitute was developed using modified starch, soybean protein isolate (SPI), emulsifiers,
water holding agents and flavoring agents. In order to maximize the emulsification stability of the fat substitute, bovine
fat/emulsion ratio, homogenization time, oil phase temperature and water phase temperature were optimized by orthogonal
array design and quadratic general rotary unitized design. As a result, the optimal process conditions were found to be 35:65 bovine
fat/emulsion ratio, 30 s homogenization time, 90 ℃ oil phase temperature, and 20 ℃ water phase temperature.
2011, Vol. 25 
