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| Changes in the Expression of Myosin and Myosin Heavy Chain Isoform Genes during the Growth of Small Tailed Han Sheep |
| XUE Baoling, SONG Xiaobin |
| 1.College of Vocational and Technical, Inner Mongolia Agricultural University, Baotou 014109, China; 2.Ulanqab Food and Drug Inspection Institute, Ulanqab 012000, China |
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Abstract We carried out this study in order to explore the pattern of change in the expression of the myosin and myosin heavy chain (MyHC) isoforms genes in Small Tailed Han sheep at different growth stages. MyHC isoforms from the Biceps femoris of naturally grazed Small Tailed Han sheep aged between 1 and 18 months were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The relative gene expression level of MyHC was investigated by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). The content of myosin in the Biceps femoris showed an upward trend with age up to 9 months. The relative expression level of the MyHCI gene showed a downward trend with age. The relative expression level of the MyHCIIa gene was positively correlated with age. The expression level of the MyHCIIb gene first decreased and then increased. The expression of the MyHCIx gene was the lowest and highest at 9 and 12 months of age, respectively. The expression of the MyHCI changed greatly between 6 and 9 months of age, indicating a rapid growth rate, but changed little between 9 and 12 months, indicating a slow growth rate.
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2022, Vol. 36 
