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| Determination of Epinephrine and Dopamine Residues in Meat and Meat Products by Solid Phase Extraction Combined with Ultra-High Performance Liquid Chromatography-Mass Spectrometry |
| HE Liangna, MA Junmei, FAN Lixin, LI Qiang |
| Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050299, China |
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Abstract An ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) method was established for the detection of the residues of two catecholamine compounds (epinephrine and dopamine) in meat and meat products. The samples were extracted with 10 mmol/L phosphate buffer solution, and the extract was purified on a WCX solid phase extraction column, and separated on a Waters Acquity BEH C18 column. The analytes were detected using an electrospray ion source in the positive ion multiple reaction monitoring (MRM) mode, and quantified by a matrix matched internal standard method. The results showed that the calibration curves for epinephrine and dopamine were linear in the concentration range of 0.5–50 ng/mL with correlation coefficient (R2) greater than 0.996. The limits of detection and limits of quantification were 0.39–0.40 and 1.00 μg/kg for livestock meat, 0.36–0.37 and 0.98 μg/kg for poultry meat, and 0.38 and 0.96–0.97 μg/kg for meat products, respectively. At spiked concentrations of 1–10 μg/kg, the recoveries of the method were between 83.7% and 111.2%, and the precision relative standard deviation (RSD) was 1.28%–5.76%. This method has the advantages of high sensitivity and accuracy, and is suitable for the determination of residual catecholamine compounds in meat and meat products.
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LING Yihan, WANG Xiaomeng, WU Shouxun, ZHOU Ting, LI Xingjie, ZHOU Heting, ZHANG Shuwen, YIN Wenya, WANG Jianping, WANG Yi. Determination of Residues of the Novel Veterinary Drug Fluralaner in Chicken Tissues and Eggs by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Meat Research, 2024, 38(10): 30-35. |
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2021, Vol. 35 
