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| Determination of 10 β-Agonists in Pork and Lamb Samples by On-Line Solid Phase Extraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry |
| MA Junmei1, SUN Lei1, LIU Zhuo2, SHEN Lu1, ZHANG Dongsheng1, JIA Wenxuan1, LI Qiang1,*, FAN Sufang1,* |
| 1.Hebei Food Inspection and Research Institute, Shijiazhuang 050000, China; 2.College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China |
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Abstract In this research, we developed a fully automated method to determine 10 β-agonists in pork and lamb using on-line solid phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS). After being enzymatically digested at 37 ℃ for 16 h, samples were purified by on-line solid phase extraction on an MCX column, and then separated on an XBridge C18 column by gradient elution using 0.1% formic acid solution and acetonitrile as the mobile phase. Mass spectrometric data were acquired under multiple reaction monitoring (MRM) mode using positive ion electrospray ionization. Quantitation was performed by the internal standard method. The results showed a good linear relationship in the concentration range of 0.01–10.00 ng/mL was achieved for all analytes with acorrelation coefficient higher than 0.999 0. The limits of detection (LOD) were in the range of 0.004–0.040 μg/kg and the limits of quantitation (LOQ) were in the range of 0.02–0.20 μg/kg. The recoveries of the method ranged from 76.5% to 107.7%, with relative standard deviation (RSD)lower than 10%. These results demonstrate that the method is simple, sensitive, and can be used for quantification of 10 β-agonists in pork and lamb samples.
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2018, Vol. 32 
