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| Determination of Sulfamethazine in Pork Using a Surface Plasmon Resonance Biosensor |
| YU Jing1, FAN Sufang1,*, LI Qiang1, MA Junmei1, SHI Weijie1, MENG Zhijuan1, ZHANG Teng2, ZHANG Yan1,* |
| 1.Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050091, China; 2.Beijing Zhonglongyicheng Technology Co. Ltd., Beijing 100071, China |
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Abstract A new method was developed to determine sulfamethazine (SM2) in pork samples based on surface plasmon resonance (SPR). As a vector, nano-gold was used to connect SM2-bovine serum albumin conjugate to the surface of an SPR biosensor chip. Antigen and antibody concentrations were optimized, and optimal calibration curve was established. The recoveries of the method were in range of 87.6%-107.4% at spike levels of 10, 20 and 100 μg/kg, with relative standard deviations (RSDs) lower than 11%. This method was used to determine SM2 in real samples, and the results were confirmed by liquid chromatography-tandem mass spectrometry. Our data indicated that the method presented in this paper could be used for rapid screening and analysis of SM2 in pork samples.
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2018, Vol. 32 
