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| Determination of Sixteen Quinolones Residues in Pork by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| ZHANG Yingying, LI Yingying |
| China Meet Research Center, Beijing Academy of Food Sciences, Beijing 100068 |
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Abstract An efficient ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was
established for the analysis of residues of 16 quinolones (danofloxacin, difloxacin, lomefloxacin, pefloxacin, enoxacin,
orbifloxacin, marbofloxacin, sparfloxacin, oxolinic acid, nalidixic acid, ofloxacin, norfloxacin, flumequine, enrofloxacin,
sarafloxacin, and ciprofloxacin) in pork. Samples were extracted with 1% acetic acid-acetonitrile, and the extract was
subjected to chromatographic separation on a weak anion solid-phase extraction column. Quantification of 16 quinolones
was achieved by UPLC-MS/MS using internal standard method. The detection limit and quantification limit of the method
were 0.2 μg/kg and 0.8 μg/kg respectively for all 16 quinolones. The average recoveries of 16 quinolones in blank pork at
three spiked levels of 2.0, 5.0 and 10.0 μg/kg ranged from 70% to 120%, with relative standard deviation (RSD) less than 6%.
The method had the advantages of fast analysis, clean sample pretreatment and less pollution. Therefore, it can be used for
the detection of quinolone residues in pork.
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