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Isolation, Identification and Growth Prediction of Dominant Spoilage Bacteria in Cold-Stored Sturgeon under Vacuum Condition |
JIAO Wei-zhen;CHEN Si-lu;ZHANG Zhi-chao;LI Ping-lan |
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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Abstract The objective of this study was to isolate and identify dominant spoilage bacteria in cold-stored sturgeon under vacuum condition, as well as make predictions of the bacteria growth. Sensory evaluation and selective culture were used to monitor the kinetic growth of dominant spoilage bacteria. Based on 16S rRNA gene sequencing, Q-1 and C-1 were identified and isolated selectively as dominant spoilage bacteria. The results showed that Aeromonas sp. and Enterobacter sp. were dominant spoilage bacteria in cold-stored sturgeon under vacuum condition. Q-1 was likely to be a new subspecies of Aeromonas veronii, and C-1 was Enterobacter ludwigii. Compared with C-1, the lag phase of Q-1 was longer and the growth rate was higher. A modified Gompertz equation could make a credible prediction of the dynamic growth of C-1 and Q-1 in the cold-stored sturgeon under vacuum condition.
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[1] |
GAO Tianqi, WANG Chao, WANG Lan, SHI Liu, CHEN Sheng, WU Wenjin, CHEN Lang, XIONG Guangquan. Isolation, Identification and Spoilage Ability of Dominant Spoilage Bacteria from Channel Catfish[J]. Meat Research, 2023, 37(3): 1-6. |
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