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| Sephadexg-25 Column Chromatographic Separation, MALDI-TOF-MS Analysis and Amino Acid Composition of Sheep Bone Collagen-derived ACE Inhibitory Peptides |
| FENG Ya-rong，MA Li-zhen，LI Li |
| 1. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China；
2. Department of Food Science, Tianjin Agricultural University, Tianjin 300384, China ；
3. College of Biology Engineering, Tianjin Tianshi College, Tianjin 301700, China |
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Abstract Sephadexg-25 column chromatography was used to separate hydrolyzed sheep bone collagen prepared with neutral protease to molecules of larger than 5000 D in size and two ACE inhibitory peptides, peaks 1 and 2, both of which had an average inhibitory rate of over 92%. MALDI-TOF-MS （matrix assisted laser desorption ionization/time of flight MS） analysis showed that both peaks consisted of more than 30 fragments and the peptide showing the highest intensity was 1077.36 D in peak 1 and 1048.47D in peak 2. The total amino acid content of peak 1 was 23.49 g/100 g and of peak 28.32 g/100 g. Eight essential amino acids were found in both of them, and three nonessential amino acids such as Gly, Pro and Ghi were also abundantly contained.
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2011, Vol. 25 
