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| Polymerase Chain Reaction Method for Detection of Listeria monocytogenes in Meat Samples |
| LI Yan;CUI Hongbin;LIU Hongyu |
| 1. Food College of Northeast Agricultural University, Harbin, China, 150030; 2. Public Health College of Harbin Medical University, Harbin, 150086) |
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Abstract Abstract: Objective:To establish a rapid, sensitive and specific polymerase chain reaction (PCR) method for detection of Listeria monocytogenes (LM). Methods:Polymerase chain reaction (PCR) technique was used to amplify the ivlA gene of Listeria monocytogenes of food specifically,value the specificity and sensitivity of this method. Results:The target gene ivlA was detected at 445bp, and only the target gene of Listeria monocytogenes was amplified while the other bacteria was not detected; this method could also detect the limit of DNA . Conclusion:The PCR method is more concise, rapid, sensitive and more specific than conventional culture method for detection of Listeria monocytogenes in meat samples. This method is useful for the investigation of Listeria in meat samples.
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2010, Vol. 24 
