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| Determination the Concentration of α-Tocopherol in Goose by HPLC with Diode Array Detector |
| ZENG Yanbing;LI Weihong;YAN Han;YIN Defeng;WEI Aihua;LUO Linguang |
| 1.Institute of Agricultural Product Quality Safety and Standards Jiangxi; 2.Academy of Agricultural Sciences,The Centre of Inspection and Testing for Meat and Meat-products,Ministry of Agriculture,Nanchang Jiangxi,China |
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Abstract Object:To determinate the concentration ofα-tocopherol in the goose using HPLC with DAD. Methods:Goose was added in anhydrous alcohol,potassium hydroxide and vitamin C.The mixture was shaken and saponified at 100℃for 30 minutes,following extracted by ether and dried by evaporation, dissolved in ethanolgrain alcohol,and analyzed by HPLC-DAD,α-tocopherol was separated on SunFire TmC18,15 cm×4.6 mm,5μm column and detected at 300nm by using methanol:water(98:2)as mobile phase when the column temperature was 30℃.Result:The linear ranges of determination ofα-tocopherol was 0.1～50.0mg/L(r=0.9985).Theα-tocopherol in goose is 0.264μg/mL Conclusion:The method is sensitive,simply and accurate.It can be used to determine the concentration ofα-tocopherol in animal food.
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2009, Vol. 23 
