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Extraction and Detection of Type Ⅰ Collagen From Skeletal Muscle of Sunit Sheep |
LI Xiaobo |
1.Department of Food Science,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China; 2.Inner Mongolia Wulanchabu Vocational College,Biotechnology Department,Inner Mongolia Wulanchabu 012000 |
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Abstract In order to establish a simple and effective method to extract,purify and detect type I collagen from skeletal muscle.Type I collagen in Sunit mutton skeletal muscle was extracted three steps crushing,acidolysing and salting out.Results showed that the obtained collagen was proved to have high electrophoretic purity.therefore,The method provides a valuable means for studying collagen and tenderness of muscle.
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