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| Effect of Resveratrol on Oxidative Stress-Induced Muscle Fiber Type Transformation in C2C12 Myotubes Based on Transcriptomics and Apoptosis Analysis |
| ZHOU Xueyu, ZUO Huixin, LIU Yunge, GONG Benzhi, LIANG Rongrong, YANG Xiaoyin, MAO Yanwei, ZHANG Yimin, ZHU Lixian |
| 1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; 2. Jining Academy of Agricultural Sciences, Jining 272031, China |
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Abstract In this study, the effect of resveratrol (Res) on the transformation of muscle fiber types in C2C12 myotubes caused by oxidative stress induced by H2O2 was investigated. Using untreated C2C12 myotubes as the control group, C2C12 myotubes were treated with 400 μmol/L H2O2 for 24 h to form an oxidative stress model group, or treated sequentially with 10 μmol/L Res for 24 h and 400 μmol/L H2O2 for 24 h to form a Res improvement model group. To investigate the effect of Res on oxidative stress-induced muscle fiber type transformation in C2C12 myotubes, reactive oxygen species (ROS) levels, apoptosis rate, muscle fiber types (such as myosin heavy chain (MyHC) I, MyHC IIa, MyHC IIx and MyHC IIb), the mRNA and protein expression levels of apoptosis-related factors (caspase-9, caspase-3, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X protein (Bax)) were measured, and transcriptomic analysis was also performed. The results showed that Res significantly increased mRNA expression in type I and IIa muscle fibers (P < 0.05), decreased mRNA expression in type IIx and IIb muscle fibers (P < 0.05), increased the protein expression of slow muscle fibers (P < 0.05) and decreased the expression of fast muscle fibers (P < 0.05). In addition, Res significantly reduced ROS levels and apoptosis rate (P < 0.05), and decreased the relative expression level of cleaved caspase-3 (P < 0.05), and increased Bcl-2/Bax ratio (P < 0.05). The results of transcriptomics showed that Res participated in apoptosis inhibition pathways such as mitochondrial apoptosis pathway. These results indicate that Res can inhibit oxidative stress, reduce the effect of oxidative stress on the transformation of muscle fiber types, increase the proportion of slow muscle fibers and decrease the proportion of fast muscle fibers through mitochondria-mediated apoptosis.
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2026, Vol. 40 
