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| Development of Ready-to-Use Quality Control Samples for Rapid Detection of Escherichia coli in Meat and Meat Products |
| ZHANG Yalun, CHEN Chen, WANG Hui, ZHANG Tonghe, ZHANG Shuang, LI Xian, LI Yongbo |
| Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China |
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Abstract This paper developed a ready-to-use quality control sample which can be directly used without secondary culture for the rapid detection of Escherichia coli in meat and meat products by matrix assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS). The sample was evaluated for homogeneity and stability. The results showed that the survival rate of the quality control sample prepared using the selected cryoprotectant was above 90%. The culture count result of the quality control sample (F = 0.567) was less than the critical value, suggesting good uniformity. The quality control sample was stable for 14 days at 37 and 25 ℃, and the resuscitation rates were 101.5% and 99.6% at ?20 and 4 ℃ for 28 days, respectively. After 28 days of storage at 37 and ?20 ℃, the results of MALDI-TOF-MS were consistent, indicating that the developed sample had good stability and met the requirements of quality control samples.
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2023, Vol. 37 
