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| Determination of Five Poppy Husk Alkaloids in Meat Products Used in the Catering Industry by QuEChERS Combined with Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Using Internal Standard Method |
| ZHOU Yingchun, YANG Shengru, HUA Xiangmei, LI Dandan |
| 1. Henan Import Meat Designated Port Luohe Inspection Area Service Center, Luohe 462300, China; 2. College of Food and Bioengineering Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China; 3. Luohe Customs of the People’s Republic of China, Luohe 462300, China |
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Abstract An analytical method using a modified QuEChERS (quick, easy, cheap, effective, rugged, safe) extraction procedure combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an internal standard was developed for the determination of five poppy husk alkaloids in meat products used in the catering industry, including morphine, codeine, nocictin, papaverine and thibaine. Samples were extracted with acetonitrile-0.1% (V/V) aqueous ammonia solution and the extract was purified with a mixture of sorbents: 2 g of anhydrous magnesium sulfate, 50 mg of primary secondary amine (PSA), and 100 mg of C18E. Complete separation of the poppy husk alkaloids was achieved on an ACQUITY UPLC? HILIC Core-shell chromatographic column (50.0 mm × 2.1 mm, 1.7 μm) using a mobile phase composed of 10 mmol/L ammonium formate and 0.1% formic acid in acetonitrile by gradient elution. Mass spectrometric data were acquired using an electrospray ionization (ESI) source in the positive ion mode with multi-reaction monitoring (MRM). The analytes were quantified by an internal standard method. The results showed that the five poppy husk alkaloids could be separated effectively within eight minutes and good linearity were obtained for morphine and codeine in the range of 0.2–20.0 ng/mL and for the other amines in the range of 1.0–100.0 ng/mL (r > 0.999). The limits of detection (LOD) and limits of quantification (LOQ) were in the range from 0.3 to 1.5 ng/mL and from 1.0 to 5.0 ng/mL, respectively. Satisfactory recoveries for spiked samples were obtained in the range of 85.8%–107.4% with relative standard deviation (RSD) lower than 4.7%. In summary, the method is easy to operate and has good purification effect and high detection efficiency. It can be applied to the rapid determination of the five poppy husk alkaloids in meat products used in the catering industry.
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2021, Vol. 35 
