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| Content s of 18 Amino Acids in Meat from 6 Species of Special Farm Animals |
| XIN Lina1, LIU Changkai1, HE Linfei1, YUAN Rongsui2, CHEN Shengjie3, WANG Jie4,* |
| 1.Guangxi-ASEAN Center for Food and Drug Safety Control, Nanning 530021, China; 2.Guangxi Zhuang Autonomous Region Food and Drug Examination and Verification Center, Nanning 530029, China; 3.College of Pharmaceutical, Guangxi Medical University, Nanning 530021, China; 4.Pharmaceutical Factory of Guangxi Medical University, Nanning 530021, China |
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Abstract In this paper, the contents of 18 amino acids in meat from 6 newly developed special farm animals, namely blue peacock, cobra, mouse snake, sea snake, sea dragon and sea star, were determined with an automatic amino acid analyzer. After protein precipitation with 90% ethanol, the samples were hydrolyzed with 6 mol/L hydrochloric acid solution for 22 h and then centrifuged. The supernatant was blown to dryness with nitrogen and the residue was re-dissolved and filtrated before being injected and analyzed. A mixture of sodium citrate and sodium hydroxide solutions was used as the mobile phase for gradient elution and fluorescence detection was performed at 440 nm for Pro and at 570 nm for 17 other amino acids. The results showed that the linear ranges were 1.6–8.0 mg/L for cystine, 2.0–10.0 mg/L for taurine, and 3.2–16.0 mg/L for other amino acids (r = 0.998 5–0.999 9). The detection limits of the method were 0.04–0.42 mg/L. Recoveries from samples spiked at 3.2 mg/L for cystine, 4.0 mg/L for taurine, and 6.4 mg/L for other amino acids were in the range between 96.11% and 109.76% with relative standard deviations (RSDs) between 0.14% and 1.62%. Recoveries from samples spiked at 6.4 mg/L for cystine, 8.0 mg/L for taurine, and 12.8 mg/L for other amino acids were in the range between 96.12% and 104.13% with RSDs were 0.11% -1.88%. The precision, stability and repeatability RSDs were 0.08%–0.94%, 0.16%–0.97% and 0.09%–0.91%, respectively. This method was rapid, accurate and reliable, and could be used to determine amino acids in blue peacock, cobra, mouse snake, sea snake, sea dragon and sea star.
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2019, Vol. 33 
