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| Determination of Methyl Testosterone Residues in Foods of Animal Origin by Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| ZHOU Yingchun1, WANG Linpei1, LIU Shaobo2, HUA Xiangmei1, LIU Huiling1, PENG Xinran1 |
| 1.Luohe Entry-Exit Inspection and Quarantine Bureau, Luohe 462300, China; 2.Cargill Food (Luohe) Co. Ltd., Luohe 462300, China |
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Abstract An efficient method for the determination of methyl testosterone residues in foods of animal origin with low matrix effect and high recovery was presented by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). Samples were extracted with acetonitrile, and the extract was cleaned up on a C18 solid phase extraction column (SPE-C18), concentrated by nitrogen gas blowing, detected by UPLC-MS-MS and quantitated by external standard method. The calibration curve for methyl testosterone exhibited a good linearity with R2 higher than 0.998. The limit of detection (LOD) was 0.3 μg/kg, and the limit of quantification (LOQ) was 1.0 μg/kg. The recoveries of methyl testosterone in spiked samples were in the range of 70.60%–112.41% at spiked levels of 1.0, 2.0, 10.0 and 40.0 μg/kg, with relative standard deviations (RSD) between 4.33% to 9.26%. This method was simple, low-cost, sensitive and suitable for the batch determination of methyl testosterone residues in foods of animal origin.
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ZHAN Hao, XUAN Lei, KONG Weiheng, LIU Wen, CHEN Ranran, MENG Xiaodi, ZHOU Xiaoying, Lü Yaning, HAN Fang, YU Xiaofeng. Rapid Determination of 12 Diuretic Residues in Pork by Modified QuEChERS Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Meat Research, 2025, 39(12): 69-75. |
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2017, Vol. 31 
