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| Simultaneous Determination of Thirteen Sulfonamides in Pork by HPLC-MS/MS |
| ZHANG Zhi-gang |
| State Key Laboratory of Food Safety Technology for Meat Products, Xiamen Yinxiang Group Co. Ltd., Xiamen 361100, China |
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Abstract An HPLC-MS/MS method was developed for the simultaneous and rapid determination of thirteen sulfonamides in pork. Sulfonamide residues in samples were extracted with acidic acetonitrile. The extract was concentrated, defatted with hexane, filtered through a 0.22μm filter membrane and transferred to a sample vial prior to HPLC-MS/MS analysis. Chromatographic separation was performed on a Kinetex C18 (100 mm × 2.1 mm, 2.6 μm) column using a gradient elution with water (0.1%formic acid) and acetonitrile as mobile phases. The mass spectrometer was operated using multiple reaction monitoring in positive ionization mode. All thirteen sulfonamides showed good linearity in the concentration range of 1 to 300μg/kg, and the limit of quantification for each of the sulfonamides was below 10μg/kg. The average recoveries from samples spiked at levels of 1, 2 and 10μg/kg ranged from 60.1%to 78.1%with relative standard deviations of 0.24%—8.88%. The HPLC-MS/MS method is simple, sensitive, selective, and applicable for the routine simultaneous determination of sulfonamides in meat.
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2013, Vol. 27 
