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| Determination of Tilmicosin and Tylosin Residues in Pork and Chicken by SPE-HPLC |
| LIU Yong-jun;JIANG Yan-bin |
| China Animal Disease Control Center, Quality Control & Inspection Center for Domestic Animal Product, Ministry of Agriculture,
Beijing 100125, China |
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Abstract An SPE-HPLC method to determine tilmicosin and tylosin residues in pork and chicken has been established.
Samples were extracted with acetonitrile and extracted antibiotic residues were cleaned up using a C18 SPE column, separated
by HPLC, detected with a PDA detector and quantified by external standard method. The established calibration curve showed
good linearity in the range of 30 to 1000 μg/L (r＞0.999). The recovery rates of tilmicosin and tylosin from samples spiked at
concentrations of 0.05, 0.10 mg/kg and 0.15 mg/kg were 72.7%–90.8%, with coefficients of variation of 2.6%–8.3%. The limits
of detection (LODs) and limits of quantification (LOQs) for both antibiotics were 30 μg/kg and 50 μg/kg, respectively. The
HPLC method was simple, rapid, accurate, precise and suitable to determine tilmicosin and tylosin residues in pork and chicken.
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2012, Vol. 26 
