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| Determination of Benzo(a)pyrene in Meat Products by HPLC with Solid-Phase Extraction |
| LIN Hui;YAN Chun-rong;XU Chun-xiang;LU Jian |
| Jiangsu Institute for Product Quality Supervision and Inspection, Nanjing 210007, China |
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Abstract A high performance liquid chromatography with solid-phase extraction method was developed for the determination
of benzo(a)pyrene in meat products. The extracted sample was purified on a Cleanert BaP solid-phase extraction column and
separated on a Waters SunFire C18 column (4.6 mm × 250 mm, 5μm) using a mobile phase composed of acetonitrile and water
(88/12) at a flow rate of 1.0 mL/min. Benzo(a)pyrene was measured using a fluorescence detector at an excitation wavelength of
386 nm and an emission wavelength of 405 nm. The result showed that benzo(a)pyrene displayed good linearity relationship
over the concentration range of 0.5－10 ng/mL, with a coefficient correlation of 0.9978. The limit of detection was 0.04 μg/kg,
and the precision (RSD) for 6 replication determinations was below 5%. The average blank spike recoveries across the levels was
in the range of 82.2%－ 94.9% (n ＝ 3). This method was rapid, simple, reproducible, and applicable for benzo(a)pyrene
determination in meat products, providing guarantee for quality control and safety evaluation of meat products.
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2012, Vol. 26 
