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| Determination of ATP-Related Compounds in Fish by RP-HPLC |
| JIA Peng-yu，SUN Rui，YU Long-hao，HE Shu-qing，ZHANG Meng，ZHANG Yu-ning |
| 1. Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Heilongjiang Bayi Agricultural
University, Daqing 163319, China；2. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural
University, Daqing 163319, China；3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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Abstract The method for determination of ATP-related compounds in fish was established. The content of ATP-related compounds in fish were determined by RP-HPLC with a Sepax Bio-Cls（250mm × 4.6mm ID, 5 lam, 200A.）column, and UV detector at a wave length of 254 nm（BW 16 nm）. Mobile phase was 60 mmol/L KH2PO4 and 60 mmol/L K2I-IPO4 （pH=6.68） and flow rate was 0.6mL/min. The separation was achieved in 20 min. All the calibration curves were linear in the mass concentration range of 1 - 400 mg/L. The average recoveries of the standard additions were between 98.3% - 104.1%, and the LOD were between 15 -40μg/L for ATP-related compounds. This method proved to be reliable and reprodcible.
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2012, Vol. 26 
