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| An Optimized Sample Pretreatment Method for Quantitative Detection of Meat Adulteration by PCR |
| YANG Jun-na，LI Jia-peng，ZHOU Tong，TIAN Han-you，QIAO Xiao-ling |
| China Meat Research Center, Beijing 100068, China |
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Abstract In this study, an L9（34） orthogonal array design was employed to optimize homogenization conditions for use in the sample pretreatment for quantitative detection of meat adulteration by PCR. Meanwhile, the effect of sample homogeneity on the accuracy of PCR detection was examined using three different sampling amounts： 30, 10 g and 3 g of rump pork. The optimal homogenization conditions were found to be： 1：4 （m/V） meat/water ratio, 12000 r/min homogenizer rotary speed and 8 min homogenization time. Under these conditions, a good homogeneity was obtained for pretreated samples, and the results of determination of sample mass, DNA concentration and real-time PCR Ct also showed a good homogeneity and no significant difference. Further, the optimized homogenization conditions were used to detect pork at the three sampling levels above, with the result that sampling amount had no effect on sample homogeneity.
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2011, Vol. 25 
