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| Determination of Fluoroquinolone Residues in Pork by HPLC |
| ZHU Li-ping，ZHU Tao，WU Na，NI Yong-fu，WANG Yong，YAN Qiu-cheng |
| Jining Import and Export Inspetion and Quaratine Bureau, Jining 272000, China |
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Abstract An HPLC with FLD （fluorescence detector） method was established for the determination of two kinds of fluoroquinolones in pork. Pork samples were extracted with 1% acetic acid/acetonitrile, followed by water removal by anhydrous sodium sulfate and clean-up by acetonitrile saturated n-hexane. The mobile phase was composed of acetonitrile and 0.01 mol/L sodium phosphate （pH 3, adjusted with phosphorous acid） at a ratio of 10：90 （V/V）. The analytes were separated on a ZORBAX-SB-CIs column and detected by a fluorescence detector. The established method proved accurate, rapidy and sensitive.
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2011, Vol. 25 
