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| Optimization of Preparation Process for Hydrolyzed Grass Carp Visceral Proteins without Adding Exogenous Enzymes |
| LIU Lan;MA Chang-wei |
| College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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Abstract Grass carp visceral waste was used as the raw material to prepare hydrolyzed animal proteins by autolysis in an
aqueous NaCl medium without the presence of exogenous enzymes. In order to achieve maximum degree of hydrolysis (DH) of
grass carp visceral waste, operating conditions such as NaCl amount, material/water ratio, temperature, holding time and pH
were optimized using orthogonal array design method. The optimal conditions for maximizing the DH of grass carp visceral
waste were material/water ratio 1:1.5, temperature 45 ℃, holding time 4 h, pH 9.0, and as a result, a DH of 77.43% was obtained.
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2011, Vol. 25 
