Abstract：This study established a high-throughput method for the simultaneous and efficient detection of amoxicillin and ampicillin residues in silver carp as a representative of freshwater fish using high performance capillary electrophoresis (HPCE) with a diode array detector. Samples were extracted with acetonitrile, concentrated by rotary evaporation and cleaned up using a solid phase extraction (SPE) column. The capillary column was washed with 30 mmol/L borax buffer solution at pH 7.85. The detection wavelength, voltage and temperature were set at 210 nm, 20 kV and 25 ℃, respectively. Baseline separation of amoxicillin and ampicillin was accomplished within 20 min. The peak area of amoxicillin and ampicillin showed a linear correlation with their concentrations in the range of 0.02–10.00 μg/mL, with correlation coefficients (R2) greater than 0.990. The recoveries of amoxicillin and ampicillin spiked in freshwater fish were 78.90%–85.72% and 75.36%-86.34%, respectively, with within-day coefficient of variation (CV) less than 8.01% and 8.06%, respectively.
孙雪峰，王方雨. 高效毛细管电泳法检测淡水鱼肉中阿莫西林和氨苄西林残留方法的建立[J]. 肉类研究, 2023, 37(6): 29-33.
SUN Xuefeng, WANG Fangyu. Establishment of a High Performance Capillary Electrophoresis Method for the Determination of Amoxicillin and Ampicillin Residues in Freshwater Fish. Meat Research, 2023, 37(6): 29-33.
2023, Vol. 37 
