Development and Application of a Real-Time Polymerase Chain Reaction Assay for Simultaneous Detection of Chicken-, Duck-, Swine- and Bovine-Derived Ingredients in Quick-Frozen Food
ZHOU Cang, SUN Xiaoxia, WANG Jinfeng, FU Qi, WANG Kairui, LIU Libing, WANG Jianchang
1.College of Public Health, Hebei Medical University, Shijiazhuang 050017, China; 2.Technology Center of Shijiazhuang Customs, Shijiazhuang 050051, China; 3.Hebei Key Laboratory of Environment and Human Health, Shijiazhuang 050017, China
Abstract：A TaqMan real-time polymerase chain reaction (PCR) assay was developed for the simultaneous detection of chicken-, duck-, swine- and bovine-derived ingredients in quick-frozen food. Specific primers and TaqMan probes were designed and synthesized using the transforming growth factor beta-3 (TGFB3) gene of chicken, the prion protein (PRNP) gene of swine and the growth hormone (GH) gene of duck and bovine as target genes. The PCR reaction systems and conditions were optimized. The developed real-time PCR assay demonstrated good specificity, in which specific amplifications were observed for chicken, duck, swine and bovine. The assay also showed high sensitivity with limits of detection for chicken-, duck-, swine- and bovine-derived ingredients of 10.0, 1.0, 10.0 and 10.0 pg/μL, respectively. The PCR assay could detect the four kinds of animal-derived ingredient in 34 commercial quick-frozen foods. Further analysis revealed that the results of the PCR assay for 15 quick-frozen foods were inconsistent with the ingredients on the label. The real-time PCR assay developed in this study has good specificity and sensitivity, and it is simple to operate and suitable for the rapid detection of animal-derived ingredients in quick-frozen foods.
周 藏，孙晓霞，王金凤，付 琦，王恺睿，刘立兵，王建昌. 同时检测速冻食品中鸡、鸭、猪和牛源性成分实时荧光聚合酶链式反应方法的建立及应用[J]. 肉类研究, 2022, 36(8): 29-34.
ZHOU Cang, SUN Xiaoxia, WANG Jinfeng, FU Qi, WANG Kairui, LIU Libing, WANG Jianchang. Development and Application of a Real-Time Polymerase Chain Reaction Assay for Simultaneous Detection of Chicken-, Duck-, Swine- and Bovine-Derived Ingredients in Quick-Frozen Food. Meat Research, 2022, 36(8): 29-34.
2022, Vol. 36 
