Abstract：This study intended to develop a method for rapid determination of taurine in pork and pork products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were ultrasonically extracted with water, added with acetonitrile to precipitate protein, and re-extracted with n-hexane to remove fat. Then the analytes were detected by HPLC-MS/MS. The matrix-matched calibration curve developed showed a good linearity within the concentration range from 1 to 1 000 ng/mL (R2 > 0.998). The average recovery for positive samples spiked with taurine at concentration levels of 20–50 mg/100 g was from 92.78% to 104.96%, with relative standard deviation (RSD) between 1.39% and 3.80%. The instrumental detection limit and quantitation limit were 0.3 ng/mL and 1 ng/mL respectively; the method detection limit and quantitation limit were 0.1 mg/100 g and 0.5 mg/100 g respectively. The method is characterized by low detection limit, as well as high recovery, sensitivity, accuracy and precision. It is simple and quick to operate and suitable for the accurate determination of taurine in pork and pork products.
周海珍，栗亚琼，王淑霞，王祎娟. 高效液相色谱-串联质谱法测定猪肉及猪肉制品中牛磺酸含量[J]. 肉类研究, 2021, 35(2): 35-40.
ZHOU Haizhen, LI Yaqiong, WANG Shuxia, WANG Yijuan. Determination of Taurine in Pork and Pork Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry. Meat Research, 2021, 35(2): 35-40.
2021, Vol. 35 
