Abstract：The aim of this study is to establish a rapid and accurate method for molecular discrimination of two squid species, Nototodarus gouldi and Dosidicus gigas. The fragments of mitochondrial 16S rRNA were amplified by polymerase chain reaction (PCR) from the genomic DNA of each squid species. Polymorphic sites were determined by multiple sequence alignments and species-specific primers were designed. Multiplex PCR was conducted for molecular identification of the two squid species with the species-specific primer pairs. Multiplex PCR results showed that specific amplicons of 400 and 229 bp were generated from D. gigas and N. gouldi, respectively. Both 400 and 229 bp amplicons were obtained from the mixtures of the two species. The sensitivity test results showed that the method could detect D. gigas adulteration at 1% in N. gouldi with a limit of detection of 0.1 ng.
2020, Vol. 34 
