Determination of Diethylstilbestrol Residues in Chicken and Duck Meat Using QuEChERS Combined with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
MO Zimei, NING Xin, CHEN Ningzhou, LIN Dan
1.Guangxi-ASEAN Center For Food and Drug Safety Control, Nanning 530021, China; 2.College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, China; 3.Guangxi Food and Drug Evaluation and Inspection Center, Nanning 530021, China
Abstract：This paper aims to establish a method for the determination of diethylstilbestrol (DES) in chicken and duck meat using QuEChERS sample preparation followed by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The homogenized sample was dispersed in water and then subjected to the extraction of the analyte with acetonitrile. The chromatographic separation were performed on a XBridge Phenyl-C18 column (150 mm × 2.1 mm, 3.5 μm) by gradient elution after dispersive solid-phase extraction purification. The qualitative and quantitative analysis of DES were operated by electrospray ionization mass spectrometry under the negative mode using multiple reaction monitoring (MRM). Acetonitrile and water were used as the mobile phase. The correlation coefficient of the calibration curve was 0. 999 9 in the concentration range of 0.50 to 20.00 ng/mL. The average recoveries at three spiked concentration levels varied from 70.4% to 81.6% with relative standard deviations (RSD) of 3.1% to 3.9%. The limits of detection (LOD, RS/N ≥ 3) and quantitation (LOQ, RS/N ≥ 10) were 0.1 and 0.4 μg/kg, respectively.
莫紫梅，宁 芯，陈宁周，林 丹. QuEChERS-超高效液相色谱-串联质谱法快速检测鸡鸭肉中己烯雌酚残留[J]. 肉类研究, 2019, 33(9): 48-52.
MO Zimei, NING Xin, CHEN Ningzhou, LIN Dan. Determination of Diethylstilbestrol Residues in Chicken and Duck Meat Using QuEChERS Combined with Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. Meat Research, 2019, 33(9): 48-52.
2019, Vol. 33 
