Abstract：This research was done in order to establish a rapid quantum dot (QD)-based immunochromatographic assay (ICA) for the simultaneous detection of four nitrofuran metabolites (3-amino-2-oxazolidinone (AOZ), 5-morpholine-methyl- 3-amino-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD) and semicarbazid (SEM)). Immunogens were synthesized by N-hydroxysuccinimide (NHS)/ 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) method, and monoclonal antibodies were prepared by cell fusion. QD microspheres were coupled with AOZ, AHD, SEM and AMOZ, respectively by one-step method using EDC and NHS to obtain quantum dot submicrobeads (QBs) probes. On this basis, a fluorescent immunochromatographic strip was prepared. The results showed that the detection limits of this test strip for AOZ, AHD, SEM and AMOZ were 0.4, 0.4, 0.5 and 0.5 μg/L, respectively. The repeatability and accuracy within and between batches were evaluated by spiked recovery experiments, and the results showed that the recovery rates were 80%–110% and 80%–100% with coefficient of variations of less than 15% for intra- and inter-batch assays, respectively. The cross-reactivity among AOZ, AHD, SEM and AMOZ was less than 0.1%. In conclusion, the combination of fluorescent immunochromatography and multivariate detection technique can be successfully applied to simultaneously detect four nitrofuran metabolites with high sensitivity, accuracy and specificity.
2019, Vol. 33 
