Determination of 20 (Fluoro) Quinolones Residues in Livestock and Poultry Meat by TurboFlow Online Cleanup-Liquid Chromatography-Tandem Mass Spectrometry
WANG Bingling1, TIAN Jixin2, XIANG Jialin1, AI Lianfeng1,*
1.Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China; 2.College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
Abstract：A new method was developed for the determination of (fluoro)quinolones ((F)QNs) residues in livestock and poultry meat using TurboFlow online cleanup (TFC)-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted with methanol-acetonitrile (1 : 1, V/V) mixture. Then the extract was purified on a Cyclone-p column. Subsequently, the analytes were separated on a Hypersil Gold C8 column before being analyzed by tandem mass spectrometry. The calibration curves showed good linearity with R2 > 0.99 in the concentration range from 0.2 to 20.0 ng/mL for cinoxacin, enrofloxacin and danofloxacin, and in the range from 0.1 to 10.0 ng/mL for 17 other (F)QNs. The limit of quantization for enrofloxacin, danofloxacin and cinoxacin was 2 μg/kg, while that for 17 other (F)QNs was 1 μg/kg. Average recoveries of the analytes fortified at three levels ranged from 85.4% to 108.2%, with relative standard deviations (RSDs) between 4.04% and 8.57%. The method was characterized by simplicity, rapidity, high recovery and good repeatability and was suitable for quantitative analysis and confirmation of (F)QNs residues in foods of animal origin.
2018, Vol. 32 
