Abstract：In this study, a quick method to identify ingredients of chicken origin in livestock and poultry meat was developed by polymerase chain reaction (PCR) and fluorescence quantitative PCR using mitochondrial DNA cytochrome C coxidase subunit Ⅰ (CO Ⅰ ) gene as target gene. The mitochondrial DNA CO Ⅰ genes from chicken, duck, goose, pig, cattle, sheep, rabbit, pigeon and quail revealed distinct loci. Based on this, a set of chicken-specific primers was designed. The results showed that the designed primers could only amplify template DNA from chicken with a cycle threshold (Ct) value of 21.78. The PCR assay had a high specificity and sensitivity (at pg level) and it could be used to detect ingredients of chicken origin in livestock and poultry meat quickly and accurately.
唐修君，樊艳凤，葛庆联，贾晓旭，张 静，刘茵茵，张小燕，王 珏，高玉时. 基于线粒体DNA COⅠ 基因鉴别畜禽肉中鸡源性成分[J]. 肉类研究, 2017, 31(8): 44-48.
TANG Xiujun, FAN Yanfeng, GE Qinglian, JIA Xiaoxu, ZHANF Jing, LIU Yinyin, ZHANG Xiaoyan, WANG Jue, GAO Yushi. Identification of Chicken Origin Ingredients in Livestock and Poultry Meat Based on Mitochondrial DNA COI Gene. Meat Research, 2017, 31(8): 44-48.
2017, Vol. 31 
