摘要以DNA条形码技术鉴别进出口监督抽检的鱼肉等水产制品的种类来源,用以判别其与申报或产品标签是否相符.分别提取鱼肉等样品的基因组DNA,以目前国际上比较公认的动物线粒体细胞色素氧化酶COⅠ基因通用引物进行PCR扩增.PCR产物经测序分析后,将得到的扩增片段序列与Genbank数据库进行序列比对,同时提交Barcoding Life DNA条形码数据库(BOLD)进行鉴定分析.本批次监督抽检的16份鱼肉、鱼丸等水产制品中除1份样品未能成功获得鱼肉COⅠPCR扩增外,其余15份样品均顺利得到种类来源鉴定,鉴定结果约有31.25％的样品与产品标签标示不符.作为一种简单、快速、有效的分子鉴定技术,DNA条形码可以直接应用于鱼肉等动物源性食品的种类鉴定.
Abstract：This paper reports the application of DNA barcoding techniques to identify whether or not the real species of
insepction samples of import and export aquatic products such as fish is in accordance with the label discriptions. Genomic
DNAs from aquatic products were extracted and amplified by PCR using the universal primer mitochondrial cytochrome C
oxidase subunit Ⅰ (CO Ⅰ) gene. The amplified sequence was aligned with the corresponding ones published in the GenBank
database and was submitted to the Barcode of Life Database (BOLD) for sequence analysis. Sixteen samples of aquatic
products including fish and fishballs were detected by the proposed technique. One of them failed to be amplified by CO Ⅰ
PCR whereas the species of the other 15 was successfully identified. Howoever, 31.25% of the identifed samples were not
the same as described on their labels. As a rapid, simple and effective molecular identification technology, DNA barcoding
can be directly applied to identify the species of meat products like fish.
2013, Vol. 27 
