Comparative Studies of Enzymatic Hydrolysis of Chicken Embryo Protein by Different Proteases
LIANG Qiong，ZHANG Pei-zheng，GAO Wei，SUN He-mei
1. College of Engineering and Technology, Haikou College of Economics, Haikou 570203, China；
2. College of Food Science and Engineering, Yunnan Agricultural University, Kunming 650201, China
Abstract：The optimal process conditions for hydrolyzing chicken embryo protein by trypsin or bromelain were determined using one-factor-at-a-time combined with orthogonal array design method. Under the optimal process conditions, the degree of hydrolysis of chicken embryo protein by trypsin and bromelain were 45.53% and 38.17%, and the resulting hydrolyates contained 20.15 mg/mL and 22.69 mg/mL soluble protein and 62.55 mg/mL and 50.26 mg/mL polypeptides, respectively. These results demonstrate that bromelain is more suitable to hydrolyze chicken embryo protein than trypsin.
2011, Vol. 25 
