Abstract：Ultrasonic pretreatment followed by sequential hydrolysis with papain and pepsin was used to extract chondroitin
sulfate from bovine pipes. The optimal pepsin hydrolysis conditions were identified by one-factor-at-a-time and orthogonal
array design methods to be pH 5.9, pepsin dosage 1.4:1000 (m/V), temperature 40 ℃ and hydrolysis time 100 min. Under these
conditions, the extraction yield of chondroitin sulfate was 25.43% with a purity of 87.96%. All quality indicators investigated
completely reached the requirements of relevant national standards for chondroitin sulfate.
2011, Vol. 25 
