Abstract:In order to screen culture media which could be low-cost and easy to prepare for Lactobacillus pentosus, MRS was selected as the model culture media. It was shown that corn steep liquor dry meal could act well when replace nitrogen source and the best concentration was 20g/L. The optimized composition was determined as follows: corn steep liquor dry meal 20 g/L, glucose 20 g/L, MnSO4 0.25 g/L, CH3COONa2 g/L.The cost could be reduced by 80% and the total viable counts can increase to 3.0×109 CFU/ml.