Abstract:Action mechanism and antimicrobial effect of tea polyphenols against the pseudomonad were studied. The minimum inhibitory concentration of tea polyphenols against the pseudomonad was studied through the flat band method and then established the antimicrobial effect of varying concentration of tea polyphenols against the pseudomonad through the film filter paper. Adoption of the biological and chemical testing research the antimicrobial mechanism of the tea polyhenols against the pseudomonad. Results showed that the minimum inhibitory concentration of tea polyphenols against the pseudomonad was 0.3%,and tea polyphenols was very strong inhibition effect for the pseudomonad. The completeness of cytoderm was destroied in the presence of tea polyphenols, to cause transude of alkaline phosphatase. And to increase the permeability of bacterial cell membrane (increase the leakage of protein and sugar) then the cytoarchitecture was destroied gradually that was the reason of the antimicrobial of tea polyphenol.