Rapid Determination of Indoleacetic Acid in Meat by Single-Step, Cheap, Effective, Rugged, and Safe (SinChERS) Purification Coupled with Gas Chromatography-Mass Spectrometry
LI Xia, GAO Xifeng, CUI Yuhua, SHEN Zhonglan, CHEN Qianqian, LI Fangfang, LI Haixia, XU Dawei, LI Xinling, LIU Yanming
Industrial Technology Foundation Public Service Platform, Key Laboratory of Supervising Technology for Meat and Meat Products, State Administration for Market Regulation, Shandong Institute for Food and Drug Control, Jinan 250101, China
Abstract：A method for the determination of indoleacetic acid in meat was established using a single-step, cheap, effective, rugged, and safe (SinChERS) purification and rapid derivatization followed by gas chromatography-mass spectrometry (GCMS). After adding water and adjusting pH to 3 by formic acid, the samples were extracted with acetonitrile, purified on a SinChERS column, and blown to dryness under nitrogen. The residue was derivatized with concentrated sulfuric acid and methanol for 1 min prior to GC-MS analysis. Quantitative was performed using the matrix external standard method. The results showed that the linear relationship of indoleacetic acid concentration and its response value was good in the range of 0.01–5.0 μg/mL, with a correlation coefficient (r2) greater than 0.999. The limit of detection was 0.003 mg/kg and the limit of quantification was 0.01 mg/kg. The average recoveries of pork, beef, and mutton samples spiked at 0.01, 0.05, and 0.10 mg/kg were 92.1% to 102.1%, with relative standard deviations (n = 6) of 2.6% to 5.7%. The method is characterized by high sensitivity and short pretreatment time, and can be used for the determination of indoleacetic acid in meat.
李 霞，高喜凤，崔玉花，申中兰，陈倩倩，李芳芳，李海霞，徐大玮，李新玲，刘艳明. SinChERS净化-气相色谱-质谱法快速测定畜肉中吲哚乙酸[J]. 肉类研究, 2025, 39(4): 11-16.
LI Xia, GAO Xifeng, CUI Yuhua, SHEN Zhonglan, CHEN Qianqian, LI Fangfang, LI Haixia, XU Dawei, LI Xinling, LIU Yanming. Rapid Determination of Indoleacetic Acid in Meat by Single-Step, Cheap, Effective, Rugged, and Safe (SinChERS) Purification Coupled with Gas Chromatography-Mass Spectrometry. Meat Research, 2025, 39(4): 11-16.
2025, Vol. 39 
