High-Throughput Sequencing Analysis of Bacterial Diversity during the Slaughter Process of Yellow-Feathered Broilers
XIAO Yapei, WANG Zaitian, SUN Zhilan, LIU Fang, WANG Daoying
1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Abstract：This study used traditional culture and high-throughput sequencing techniques to analyze the status of microbial contamination on the surface of chicken carcasses and that of pre-cooled water during the slaughter process of yellowfeathered broilers, including plucking, evisceration, pre-cooling, and packaging. The results demonstrated that the total bacterial count of chicken carcasses after plucking, evisceration, and pre-cooling were 4.82, 5.03, and 4.68 (lg (CFU/g)), respectively, indicating that the slaughtering process did not achieve good decontamination; instead, there was severe microbial contamination in the carcasses after slaughter. High-throughput sequencing analysis showed that at the genus level, the dominant spoilage bacteria on the surface of broiler carcasses and in pre-cooled water during slaughter and processing were Moraxella, Pseudomonas, Staphylococcus, Lactobacillus, Macrococcus, Psychrobater, and Acinetobacter. Principal component analysis (PCA) showed no significant difference among primary and secondary pre-cooled water, pre-cooled chicken carcasses, and packaged chicken carcasses in terms of bacterial community composition at the genus level, but a significant difference between them and plucked carcasses and eviscerated carcasses. This indicates that the composition of bacteria contaminating pre-cooled water determines that on the slaughtered carcasses. Considering the changes in the composition and abundance of bacteria contaminating yellow-feathered broiler carcasses during the slaughter process, control approaches are needed to ensure the quality and safety of yellow-feathered broiler products.
肖亚培，王在天，孙芝兰，刘 芳，王道营. 基于高通量测序的黄羽肉鸡屠宰过程中菌群多样性分析[J]. 肉类研究, 2024, 38(3): 1-9.
XIAO Yapei, WANG Zaitian, SUN Zhilan, LIU Fang, WANG Daoying. High-Throughput Sequencing Analysis of Bacterial Diversity during the Slaughter Process of Yellow-Feathered Broilers. Meat Research, 2024, 38(3): 1-9.
2024, Vol. 38 
