Preparation, Isolation and Purification of Antioxidant Peptides from Chinese Giant Salamander Skin Collagen
ZHOU Yanhua, JIA Xiuqi, LI Tao, WANG Jianwen, LUO Qinghua
1. Changsha Environmental Protection Voctional College, Changsha 410004, China; 2. Zhangjiajie Golden Salamander Biological Product Development Co. Ltd., Zhangjiajie 427000, China; 3. Hunan Institute of Commodity Quality Inspection, Changsha 410111, China; 4. College of Biology and Chemical Engineering, Changsha University, Changsha 410022, China
Abstract：In order to efficiently utilize giant salamander skin as a processing by-product, the preparation, purification and purification of antioxidant peptides from giant salamander skin collagen were studied. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the antioxidant activity. The optimal process conditions that provided maximum DPPH free radical scavenging activity (40.2%) were determined as follows: enzyme dosage 8 000 U/g, pH 9.0, temperature 55 ℃, and hydrolysis time 4 h. Three fractions, P1(> 6 kDa), P2 (3–6 kDa) and P3 (< 3 kDa), were separated from the resultant hydrolysate by ultrafiltration. P3 had strong antioxidant activity, scavenging 56.7% of DPPH free radicals. P3 was further separated by gel permeation chromatography on Sephadex G-25 into three fractions, SP1, SP2 and SP3. SP3 had strong antioxidant activity with scavenging percentage of 72.3% against DPPH free radicals. Finally, the amino acid composition of SP3 was analyzed by an automatic amino acid analyzer to explore its relationship with the antioxidant activity of SP3. It was found that SP3 was rich in glycine and proline along with lesser amounts of cystine. In addition, SP3 contained significant amounts of amino acids with antioxidant activity such as alanine, arginine, glutamate and leucine, which may be responsible for the antioxidant activity of SP3.
周艳华，郏修齐，李 涛，王建文，罗庆华. 大鲵皮胶原抗氧化肽的制备及分离纯化[J]. 肉类研究, 2023, 37(12): 1-6.
ZHOU Yanhua, JIA Xiuqi, LI Tao, WANG Jianwen, LUO Qinghua. Preparation, Isolation and Purification of Antioxidant Peptides from Chinese Giant Salamander Skin Collagen. Meat Research, 2023, 37(12): 1-6.
2023, Vol. 37 
