Abstract：This study was devised to investigate the bactericidal effect and mechanism of ultrasound (US) in combination with heat (HT) against Staphylococcus aureus planktonic and biofilm cells. The results showed that US combined with HT at 70 ℃ (UH70) significantly reduced the counts of Staphylococcus aureus planktonic and biofilm cells (P < 0.05). Scanning electron microscopy (SEM) showed that UH70 obviously damaged the cell membrane. Confocal laser scanning microscopy (CLSM) indicated that UH70 sharply increased the permeability of the cell membrane, thereby causing a significant increase in the extracellular concentrations of ATP, nucleic acid and protein (P < 0.05). In addition, UH70 significantly decreased the activity of intracellular respiratory chain dehydrogenase and the concentration of extracellular polysaccharide in the biofilm (P < 0.05). In conclusion, US combined with HT could have stronger bactericidal effect than either alone. The synergistic bactericidal mechanism may be related to destroying the cell membrane, altering cell membrane permeability, causing the leakage of ATP, nucleic acid and protein out of the cells, and reducing the activity of respiratory chain dehydrogenase.
孙晋跃，王德宝，孙芝兰，熊 强，刘 芳，徐为民，王道营，李坤鹏. 超声结合热处理对金黄色葡萄球菌及其生物膜的抗菌和抗生物膜机制[J]. 肉类研究, 2021, 35(10): 62-69.
SUN Jinyue, WANG Debao, SUN Zhilan, XIONG Qiang, LIU Fang, XU Weimin, WANG Daoying, LI Kunpeng. Antibacterial and Antibiofilm Mechanism of Ultrasound in Combination with Heat against Staphylococcus aureus. Meat Research, 2021, 35(10): 62-69.
2021, Vol. 35 
