Simultaneous Determination of 11 Quinolone Residues in Livestock and Poultry Meat by QuEChERS and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
HUANG Kun, WU Wanqin, LUO Tong, FU Wenwen, FAN Xiaolong, MA Yi, WANG Huixia
Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, China
Abstract：A method for simultaneous determination of enrofloxacin, ciprofloxacin, ofloxacin, pefloxacin, norfloxacin, lomefloxacin, sarafloxacin, difloxacin, sparfloxacin, flerofloxacin and danofloxacin residues in livestock and poultry meat by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. Samples were homogenized and extracted with 2% formic acid-acetonitrile. After centrifugation, the supernatant was purified by the QuEChERS procedure, concentrated by a rapid solvent evaporation system, separated on a Waters BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with a mobile phase consisting of 0.1% formic acid-water and 0.1% formic acid-acetonitrile by gradient elution, detected in the multiple reaction monitoring (MRM) mode using an electrospray ionization source (ESI+) and quantified by an internal standard method. The results showed that good linearity was observed for all analytes in the concentration range of 2.0–80.0 ng/mL with correlation coefficients greater than 0.99 and the detection and quantitation limits were respectively 0.5–2.0 and 1.5–6.0 μg/kg. The average recoveries of blank samples spiked at 10, 25 and 50 μg/kg were 74.52%–106.83%, with relative standard deviation (RSD) values of 1.8%–8.7%(n = 6). This method proved to be simple, accurate, highly sensitive and suitable for the simultaneous determination of quinolone residues in livestock and poultry meat.
黄 坤，吴婉琴，罗 彤，付文雯，范小龙，马 弋，王会霞. QuEChERS-超高效液相色谱-串联质谱法同时测定畜禽肉中11 种喹诺酮类兽药残留[J]. 肉类研究, 2019, 33(3): 40-45.
HUANG Kun, WU Wanqin, LUO Tong, FU Wenwen, FAN Xiaolong, MA Yi, WANG Huixia. Simultaneous Determination of 11 Quinolone Residues in Livestock and Poultry Meat by QuEChERS and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Meat Research, 2019, 33(3): 40-45.
2019, Vol. 33 
