Abstract：The objective of this study was to optimize the chelating process for composite microelement iron supplement prepared from pig blood by response surface methodology. By using one-factor-at-a-time method, pH value, peptide concentration, and volume ratio of peptide solution to FeCl2 solution (1.0 mol/L) were identified as factors that significantly influence Fe2+ chelating efficiency. Subsequently, three-dimensional response surface plots were generated using a threevariable three-level Box-Behnken design to investigate the interaction among the three factors. The optimized conditions were established as follows: pH 5.40, peptide concentration 2.27%, and volume ratio of peptide solution to FeCl2 solution (1.0 mol/L) 2.16:1. Under these conditions, the predicted Fe2+ chelating efficiency was 79.37%, and the actual data was 79.41%.
2018, Vol. 32 
