Preparation and Properties of Fluorescent Immunochromatographic Test Strip for Detection of Phenylethanolamine A
GUO Huican1, LI Junhua2, WANG Lijun1, LI Chunsheng2,*
1.College of Food and Drug Engineering, Shijiazhuang University of Applied Technology, Shijiazhuang 050000, China; 2.Biology Institute of Hebei Academy of Sciences, Shijiazhuang 050000, China
Abstract：A rapid, sensitive and specific immunofluorescence method to detect phenylethanolamine A (PEAA) in urine from farm animals was developed. The diazotization method was used to conjugate PEAA to bovine serum albumin (BSA), yielding an artificial antigen. Furthermore, a specific anti-PEAA monoclonal antibody was obtained by immunization, fusion, and limiting dilution analysis. Finally, a fluorescent immunochromatographic test strip was prepared by coupling quantum dot microspheres with PEAA. The results showed that the detection limit of the test strip was 0.496 μg/L. The recovery was between 80% and 120%, and the inter and intra-batch coefficients of variation (CVs) were both less than 10%. The crossreactivity with other analogues was less than 5%. The specificity was good. Accordingly, the immunofluorescence assay was feasible for the detection of PEAA with good sensitivity, accuracy and specificity and could have wide application prospects.
郭会灿，李君华，王丽君，李春生. 苯乙醇胺A荧光免疫层析试纸条的制备及特性[J]. 肉类研究, 2019, 33(1): 55-59.
GUO Huican, LI Junhua, WANG Lijun, LI Chunsheng. Preparation and Properties of Fluorescent Immunochromatographic Test Strip for Detection of Phenylethanolamine A. Meat Research, 2019, 33(1): 55-59.
2019, Vol. 33 
