Development of a Multiplex Fluorescence Quantitative Polymerase Chain Reaction Assay for Detecting Added Genetically Modified Ingredients Derived from Plants in Meat Products
SHAO Biao1, ZHOU Xiaolan2, WANG Linlin1, CHEN Gang1,*
1.Nantong Products Quality Supervision and Inspection Institute, Nantong 226011, China; 2.Analysis and Testing Center, Nantong University, Nantong 226019, China
Abstract：Plant-derived components are often added to meat products during processing to improve their quality. The commercialization and wide circulation in the market of genetically modified crops bring increasing risk of introducing genetically modified components into meat products. In this study, a multiplex fluorescence quantitative polymerase chain reaction (PCR) assay was described for detecting added genetically modified ingredient derived from plants in meat products. Specific primers and Taq man probes targeting the CaMV 35S promoter, NOS terminator and neomycin phosphotransferase Ⅱ (NPTⅡ) marker gene were designed and the pRⅠ 101-AN DNA vector was used as template. The sensitivity, repeatability and accuracy of the multiplex PCR assay were confirmed by comparison with traditional PCR. Consistent results were obtained using the two methods.
2018, Vol. 32 
