Optimization of Extraction Conditions for the Determination of Volatile N-Nitrosamines in Meat Products Using Solid-Phase Microextraction Combined with Gas Chromatography with Nitrogen-Phosphorus Detection
ZHANG Tian1, FAN Xiaopan2, XIONG Fengjiao2, WANG Na2,*, YANG Hua2, MA Lizhen2
1.College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China;2.College of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384, China
Abstract：Nine volatile N-nitrosamines in meat products were extracted by solid-phase microextraction (SPME) and determined by gas chromatography with nitrogen-phosphorus detection (GC-NPD). The optimum extraction conditions were established as follows: using PDMS/DVB/CAR fiber, equilibrium time 10 min, extraction time 30 min, extraction temperature 40 ℃, stirring speed 400 r/min, concentration of NaCl 0.36 g/mL, and desorption time 3 min. The linear correlation coefficients for the nine analytes were 0.994 9?0.999 7. The limits of detection were 0.01?10.00 ng/mL, and the limits of quantification were 0.03?33.00 ng/mL. The recoveries of the presented method were in the range of 50.19%?93.20%, with relative standard deviation (RSD) of 2.29%?9.65%. In conclusion, the method could be used for the determination of N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodipropylamine (NDPA), and N-nitrosodibutylamine (NDBA) in meat products.
张 甜，樊晓盼，熊凤娇，王 娜，杨 华，马俪珍. SPME-GC-NPD法测定肉制品中挥发性N-亚硝胺的条件优化[J]. 肉类研究, 2017, 31(7): 50-56.
ZHANG Tian, FAN Xiaopan, XIONG Fengjiao, WANG Na, YANG Hua, MA Lizhen. Optimization of Extraction Conditions for the Determination of Volatile N-Nitrosamines in Meat Products Using Solid-Phase Microextraction Combined with Gas Chromatography with Nitrogen-Phosphorus Detection. Meat Research, 2017, 31(7): 50-56.
2017, Vol. 31 
