Species Authentication of Luchuan Pig Meat and Meat Products Using ISSR Molecular Markers
HE Yu-ling;HUANG Li;FENG Yi;WANG Miao;TENG Jian-wen
1. Institute of Light and Food Engineering, Guangxi University, Nanning 530004, China;
2. Faculty of Preclinical Medicine, Guangxi University of Chinese Medicine, Nanning 530001, China
Abstract：In this study, species identification of Luchuan pig meat and meat products was carried out by DNA fingerprint profiling of meats from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs using ISSR (inter-simple sequence repeat) molecular markers. The results showed that 3 clear and repeatable primer pairs WM12, WM27 and WM10 were selected from 34 primers. WM12 was useful for discriminating fresh and cured meat from purebred Luchuan and hybrid Luchuan pigs, while Luchuan pigs could be distinguished from Bama miniature pigs by using WM27, and from Huangjiang miniature pigs by using WM10. In conclusion, the ISSR method enables rapid and accurate identification of fresh meat and meat products from purebred Luchuan, hybrid Luchuan, Bama miniature and Huangjiang miniature pigs.
2014, Vol. 28 
