Abstract:Ujumqin sheep, aged from 1 to 18 months, were employed in this study. The typeⅢcollagen of intramuscular connective tissue was extracted and identified by SDS-PAGE, also three major amino acids were determined by high performance liquid chromatography (HPLC), and then the content of type Ⅲ collagen was calculated based on hydroxyproline. The changes of typeⅢcollagen in intramuscular connective tissue during growth of Ujumqin sheep were analyzed by SAS software. The results showed that the glycine and proline contents in typeⅢcollagen in intramuscular connective tissues were decreased significantly with increasing age of Ujumqin sheep (P < 0.05), whereas the hydroxyproline content was increased significantly (P<0.05). In addition, the content of typeⅢcollagen in intramuscular connective tissues showed a significant increase with the age of Ujumqin sheep (P<0.05).